Study the Efect of Retinoeic Acid and Mitogenes on Lens and Optic Vesicle of Mouse Embryo(Nmri) and Fos Protein Expression In Culture Medium

Purpose: This work studies possible role of FOS proteins, retinoic acid (RA) and mitogens during optic vesicle development in vitro. Materials and Methods: Fos was detected as a product of 55-62 kDa in optic vesicles via electrophoretic procedures. Incubation (24hr) of optic vesicles with RA 25nM and mitogens(serum10%,insulin2.5%) on the day 9.5 of gestation revealed that Fos was induced by mitogens like serum and insulin. Results: The results suggested that the regulation of fos may be required for cell proliferation and differentiation in normal eye development. Fos induction was inhibited by retinoic acid. Conclusion: the results showed that retinoic acid prevents vesicles growth and reduces rate of protein significantly in all treatment groups and that in addition to Fos mitogens are essential to growth of vesicles.